Investigation of Genetic Relatedness of Ten Syngonium Cultivars using RAPD markers

For the first time, genetic variability and differentiation among Ten Synconium white butterfly populations originating from tissue cultured plants maintained in pro-trays under the poly house & green house conditions in Indo American hybrid Seed Company, Bangalore were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity. The ornamental crops Syngonium generate substantial revenue worldwide in the trade of ornamentals. The understanding in the pattern of genetic diversity of these plants has important implications in breeding programs and in the improvement of plant species. Initially RAPD analysis was used to assess the level of polymorphism, similarities and relationships among Syngonium populations collected from IAHS. RAPD analysis was done by screening 10 varieties of Syngonium using four primers. Totally 10,12, 23 and 25 polymorphic bands were observed in following four primers such as E20, C02, C05 and D18. Maximum and minimum bands were amplified in E20 (80 bands) CO2 (40) primers. While, the number of faded bands also increased in both primers. Based upon the E20 and D18 primer similar as well as maximum polymorphic bands also been observed. Cluster analysis was done based on the UPGMA method using NTSYS software. Dendrogram analysis clearly noticed the Similarity Co-efficient for cultivars in the cluster I, II and II varied from 0.10-0.33, 0.781.0, 0.20-0.80 and 0.20-0.90 for E20, CO2, CO5 and E18 respectively.